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Image Search Results
Journal: International journal of pharmaceutics
Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines
doi: 10.1016/j.ijpharm.2012.12.011
Figure Lengend Snippet: PCR primers used in real-time PCR assay of transporter expression.
Article Snippet: Polyclonal canine PepT1 and
Techniques: Real-time Polymerase Chain Reaction, Expressing
Journal: International journal of pharmaceutics
Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines
doi: 10.1016/j.ijpharm.2012.12.011
Figure Lengend Snippet: Determination of mRNA levels of endogenous influx transporters, canine PepT1 (cPepT1) and canine PepT2 (cPepT2), and exogenous efflux transporters, human P-gp (hMDR1), human MRP2 (hMRP2) and human BCRP (hBCRP) in various transfected MDCKII cell lines with different passage number using real-time PCR: A, MDCKII-MDR1 cells; B, MDCKII-MRP2 cells; C, MDCKII-BCRP cells. Each bar represents mean ± SD (n = 3). *P < 0.05 and **P < 0.01 compared with the lowest cell passage.
Article Snippet: Polyclonal canine PepT1 and
Techniques: Transfection, Real-time Polymerase Chain Reaction
Journal: International journal of pharmaceutics
Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines
doi: 10.1016/j.ijpharm.2012.12.011
Figure Lengend Snippet: Western blot analysis of endogenous (canine) PepT2 (A) and PepT1 (B) in wild-type and transfected MDCKII cell lines: lane 1, MDCKII-wt (Pg-14); lane 2, MDCKII-wt (Pg-30); lane 3, MDCKII-MDR1 (Pg-5); lane 4, MDCKII-MDR1 (Pg-30); lane 5, MDCKII-MRP2 (Pg-8); lane 6, MDCKII-MRP2 (Pg-30); lane 7, MDCKII-BCRP (Pg-4); lane 8, MDCKII-BCRP (Pg-30).
Article Snippet: Polyclonal canine PepT1 and
Techniques: Western Blot, Transfection